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Key Resources Table
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Key Resources Table

Journal: Current biology : CB

Article Title: Oriented cell divisions are not required for Drosophila wing shape

doi: 10.1016/j.cub.2019.01.044

Figure Lengend Snippet: Key Resources Table

Article Snippet: The information for all fly lines is described in the . table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-wingless DSHB RRID: AB_528512 Anti-Cleaved Caspase Cell Signaling 9658P Anti-E-cad DSHB RRID: AB_528120 Chemicals, Peptides, and Recombinant Proteins Paraformaldehyde Fisher Scientific Cat# T353–500 Hoechst Invitrogen Cat# H3570 Grace’s Insect Medium Sigma-Aldrich Cat# G9771 20-hydroxyecdysone Sigma-Aldrich Cat# H5142–5MG BisTris Sigma-Aldrich Cat# B4429–25G Fetal Bovine Serum Thermo-Fisher Cat# 10082147 Pen Strep Thermo-Fisher Cat# 15070063 Experimental Models: Organisms/Strains Fly: Ubi-Ecad:GFP [ 34 ] N/A Fly: Jupiter:RFP This manuscript N/A Fly: zip:GFP BDSC Stock# 51564 Fly: dachs:GFP [ 31 ] N/A Fly: mud 4 /FM7i BDSC Stock# 9563 Fly: UAS-RNAi mud BDSC Stock# 38190 Fly: w;P(w[+mC]=Raeppli-CAAX)67E BDSC Stock# 55083 Fly: nubbin-Gal4 UAS-dcr2 [ 35 ] N/A Software and Algorithms Matlab2016b Mathworks Inc., USA http://mathworks.com Fiji [ 36 ] https://fiji.sc ImSAnE matlab script [ 33 ] https://media.nature.com/original/nature-assets/nmeth/journal/v12/n12/extref/nmeth.3648-S2.zip ilastik [ 37 ] http://ilastik.org/index.html Prism 6.0 GraphPad https://www.graphpad.com/scientific-software/prism/ , RRID: SCR_002798 R ver.3.4.1 [ 38 ] https://www.r-project.org TissueAnalyzer [ 39 ] https://grr.gred-clermont.fr/labmirouse/software/WebPA/index.html TIssueMiner [ 17 ] https://github.com/mpicbg-scicomp/tissue_miner Spindle Orientation script This manuscript https://github.com/alegoth/Spindle-Orientation-Analysis Others 35mm petri dish MatTek corporation P35G-0–14-C Cyclopore polycarbonate membrane GE healthcare Cat# 7060–2513 Leica SP8 confocal Leica Microsystems https://www.leica-microsystems.com/ UltraView Spinning disc confocal PerkinElmer http://www.perkinelmer.com/lab-products-and-services/cellular-imaging/confocal-imaging.html Open in a separate window caption a8

Techniques: Recombinant, Software, Membrane